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Biological Properties of a Polyene Antifungal Substance (AF1) Produced from Streptomyces sp. WCM-9

Woon-Seob Shin,Seunho Jung,Dong-Heui Yi,Kyoung-Ho Lee,Soo Kie Kim,Joo-Young Park,Choon-Myung Koh
Epub 2016 February 25

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Abstract



BACKGROUND: Pathogenic fungi infect humans, especially immunocompromised patients, with superficial or deeply invasive pattern. In the past 20 years, fungal infections have been increased dramatically resulted by increment of organ transplantation, cancer, AIDS patients, or use of broad-spectrum antibacterial agents. Fungal infections are now important causes of morbidity and mortality of hospitalized patients. but there is no effective antifungal antibiotics as well as antibacterial antibiotics.


OBJECTIVE: Effective new antifungal antibiotics are needed for the treatment of mycosis. So in an effort to develop effective antifungal antibiotics, we screened over 600 isolates of Streptomyces sp. from soil.


METHODS: Antifungal producing strain was selected using disk diffusion method, An antifungal substance (AF1) was purified with ethyl acetate extraction, silica gel column chromatography and reverse phase HPLC. MICs of AF1 were detected by agar dilition method.


RESULTS: The compound showed UV maxima of 307, 321, 340, 359 nm indicating methylpentaene. Minimum inhibitory concentrations of the AF1 were 3.7 μg/ml against mold, and 3.7~7.4 μg/ml against Candida species. AFI was also active against Crytococcus neoformans, with MIC of 0.9 μg/ml. The concentration of AF1 for K+ ion release from human red blood cell and hemolysis were 5 μg/ml.


CONCLUSION: The antibiotic purified from culture broth of Streptomyces sp. WCM-9 was a polyene antifungal antibiotic which have broad spectrum antifungal activity.



Keywords


Antifungal antibiotics Polyene Streptomyces




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