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Effects of Culture Condition on Secretion of Phospholipase from Candida albicans

Woon-Seob Shin,Kyoung-Ho Lee,Joo Young Park,Choon-Myung Koh
Epub 2016 February 25

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Abstract



BACKGROUND: The dimorphic yeast, Candida albicans, is considered as a dangerous opportunistic pathogen in immunocompromised hosts. Several phospholipases of C. albicans are known to be secreted into the culture medium. Phospholipases have been proposed as a virulence factor in the pathogenesis of Candida infections.


OBJECTIVE: In order to investigate enzyme production, we examined culture condition of secreted phospholipase production from C. albicans.


METHODS: C. albicans ATCC 10231 was cultivated in various media at 37℃ for 3 days. Phospholipase activity was measured by fatty acid soap precipitation in plate containing 0.04% lecithin, 0.1 M citrate buffer, pH 4.2 and 1.5% noble agar.


RESULTS: Phospholipase was highly induced when C. albicans was cultivated in broth medium (containing glucose 2%, albumin 0.2% and Fe++ ion 0.01%) and Saboulaud's dextrose agar supplemented with 0.01% sodium deoxycholate.


CONCLUSION: Highly induction of secreted phospholipase by albumin from C albicans may be play an important role in tissue invasion in the pathogenesis of C. albicans.



Keywords


Candida albicans Phospholipase Production




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