Abstract

BACKGROUND: The species of dermatophytes have been identified and classified by morphological and biochemical characterization as well as by mating experiments. But these techniques are either time consuming or lacking specificity. Recently molecular analysis has been introduced to the field of medical mycology.

OBJECTIVE: We investigated the phylogeny and taxomomy of the dermatophytes using sequence analysis of the chitin synthase 1 (CHS1) gene.

METHODS: 15 species of dermatophytes (6 strains of T.rubrum, 4 strains of T. mentagrophytes subtypes, M. canis, M. gypseum, E. floccosum, T. verrucosum, and T. tonsurans) were cultured on Sabouraud dextrose broth and their DNA were extracted by bead-beating method. Cloning and sequencing of PCR product were done.

RESULTS: The size of specific bands among dermatophytes was 615 bp in CHS1 gene. Phylogenetic analysis of sequences revealed that 6 strains of T. rubrum showed genetically identical pattern in intraspecies, but subtypes of T. mentagrophytes were different. The other dermatophytes showed different pattern in interspecies.

CONCLUSION: The phylogenetic analysis of CHS1 gene provided useful information for classification and understanding the evolution of dermatophytes species. 

Keywords

CHS1 gene Dermatophytes

KJMM 2000 June;5(2):51-59(9). Epub 2016 February 22
Copyright © 2000 by Korean Journal of Medical Mycology

Language

Korean/English

Author

Moo Kyu Suh; Department of Dermatology, College of Medicine, Dongguk University, Kyounju, Korea
Byung Chun Kim; Department of Dermatology, College of Medicine, Keimyung University, Taegu, Korea
Jung Chul Kim; Department of Immunology, Kyungpook National University School of Medicine, Taegu, Korea

Corresponding

Moo Kyu Suh, Department of Dermatology, College of Medicine, Dongguk University, Kyounju, Korea.

Publication history

Acknowledgements

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

Moo Kyu Suh

Department of Dermatology, College of Medicine, Dongguk University, Kyounju, Korea

Byung Chun Kim

Department of Dermatology, College of Medicine, Keimyung University, Taegu, Korea

Jung Chul Kim

Department of Immunology, Kyungpook National University School of Medicine, Taegu, Korea

Since epub date 2016 February 22