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Enhancement of Human Monocyte Activities against Aspergillus fumigatus by Interleukin-15

Jeong Ho Cho,Kyung Hee Chang,Hyo Youl Kim,Young Goo Song,Chang O Kim,Yoon Soo Park,Sung Kwan Hong,Aejung Huh,Joon Sup Yeon,June Myung Kim
Epub 2016 February 22

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Abstract



Background: Despite recent advances in antifungal chemotherapy, invasive aspergillosis remains an important cause of morbidity and mortality in immunocompromised patients. Interleukin-15 (IL-15) is a cytokine that is known to enhance antifungal activities of monocytes against Candida albicans.


Objective: The purpose of this study is to investigate the potentials of IL-15 to enhance antifungal activities of monocytes against Aspergillus fumigatus.


Methods: Peripheral blood monocytes from healthy adults were incubated with 0, 1, 10, 100 ng/ml of IL-15 for 1, 2, and 4 days. Then, the ability of IL-15 to elicit the production of superoxide anion, the damage of hyphae by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay, and the killing ability of Aspergillus fumigatus conidia was investigated.


Results: Incubation of peripheral blood monocytes with 100 ng/ml of IL-15 enhanced hyphal damage after 2 days (p<0.05), conidicidal activity from the first day (p<0.05), and increased the production of superoxide anion (O2–) in response to phorbol myristate acetate.


Conclusion: Our results indicate that IL-15 augments the microbicidal activity of human monocytes against Aspergillus fumigatus.



Keywords


IL-15 Aspergillus fumigatus Monocyte




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