Abstract

Background: There are three kinds of diseases caused by dematiaceous fungi: chromoblastomycosis, phaeohyphomycosis, and eumycotic mycetoma. The dematiaceous fungi have been identified and classified by morphological, biochemical and physiological tests. Recently molecular analysis has been introduced to the field of medical mycology.

Objective: We investigated the genetic diversity of dematiaceous fungi using random amplified polymorphic DNA (RAPD).

Methods: The dematiaceous fungal strains studied were eight clinical isolates of chromoblastomycosis and phaeohyphomycosis agents (3 strains of Fonsecaea pedrosoi, 2 strains of Exophiala dermatitidis, 1 strain of Exophiala jeanselmei, 1 strain of Phialophora verrucosa, 1 strain of Rhinocladiella aquaspersa) and 4 standard strains (F. pedrosoi IFM 4889, E. dermatitidis IFM 4828, P. verrucosa IFM 4928, R. aquaspersa IFM 4930). Total twelve strains of dematiaceous fungi were cultured on Sabouraud's dextrose broth and their DNA were extracted by bead-beating method.

Results: The optimal condition for PCR was template DNA 0.025 μg and annealing temperature 39℃. The RAPD analysis using OPA 10 primer (5'-GTGATCGCAG-3') of Operon kit showed different patterns among dematiaceous fungi. But one clinical isolate of F. pedrosoi showed intra-specific variability.

Conclusion: The RAPD analysis is considered a rapid and reliable method for identification and classification of dematiaceous fungi if the procedure is carefully standardized with adequate primer.

Keywords

Dematiaceous fungi Random amplified polymorphic DNA

KJMM 2003 March;8(1):7-15(9). Epub 2016 February 20
Copyright © 2003 by Korean Journal of Medical Mycology

Language

Korean/English

Author

Moo Kyu Suh; Department of Dermatology, College of Medicine, Dongguk University, Kyongju, Korea
Jin Chun Suh; Fatima Hospital, Taegu, Korea
Jung Chul Kim; Department of Immunology, Kyungpook National University, School of Medicine, Taegu, Korea
Ho Chung Lee; Department of Dermatology, College of Medicine, Dongguk University, Kyongju, Korea

Corresponding

Moo Kyu Suh, Department of Dermatology, College of Medicine, Dongguk University, Kyongju, Korea. Tel: (054) 770-8269, Fax: (054) 773-1581, e-mail: mksuhmd@hanmail.net

Publication history

Acknowledgements

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

Moo Kyu Suh

Department of Dermatology, College of Medicine, Dongguk University, Kyongju, Korea

Jin Chun Suh

Fatima Hospital, Taegu, Korea

Jung Chul Kim

Department of Immunology, Kyungpook National University, School of Medicine, Taegu, Korea

Ho Chung Lee

Department of Dermatology, College of Medicine, Dongguk University, Kyongju, Korea

Since epub date 2016 February 20