Abstract

Background: Voriconazole is a potent new triazole antifungal agent expected to be particularly useful for the treatment of invasive aspergillosis. However, in vitro susceptibility of voriconazole for clinical strains of Aspergillus species isolated in Korea has not been fully surveyed.

Objective: We determined minimum inhibitory concentrations (MICs) of voriconazole for clinical Aspergillus isolates.

Methods: A total of 100 clinical isolates of Aspergillus species (40 A. fumigatus, 24 A. flavus, 17 A. niger, 17 A. terreus and 2 A. nidulans) was tested. In vitro voriconazole susceptibility testing was accomplished utilizing the National Committee for Clinical Laboratory Standards (NCCLS) broth microdilution method M38-A. MIC of voriconazole was determined using RPMI medium at 48 h of incubation.

Results: Among the 100 isolates of Aspergillus species tested, 98% were inhibited by ≤ 1 μg/mL of voriconazole. The MICs of voriconazole ranged from 0.125 to 2 μg/mL (geometric mean MIC, 0.52 μg/mL). The MIC50 (MIC at which 50% of the isolates tested were inhibited) and MIC90 were 0.5 and 1.0 μg/mL for all Aspergillus species, respectively. The strains showing MIC≥2 μg/mL were 0/40 (0%) in A. fumigatus, 1/24 (4%) in A. flavus, 1/17 (6%) in A. niger, 0/17 (0%) in A. terreus, and 0/2 (0%) in A. nidulans.

Conclusion: These data demonstrate promising in-vitro activity of voriconazole against clinical strains of Aspergillus species isolated from Korean patients.

Keywords

Aspergillus Voriconazole Antifungal susceptibility test

KJMM 2004 September;9(3):166-173(8). Epub 2016 February 20
Copyright © 2004 by Korean Journal of Medical Mycology

Language

Korean/English

Author

Jong Hee Shin; Departments of Laboratory Medicine, Chonnam National University Medical School, Gwangju, Korea
Mira Park; Departments of Laboratory Medicine, Chonnam National University Medical School, Gwangju, Korea
Jung Won Song; Departments of Laboratory Medicine, Chonnam National University Medical School, Gwangju, Korea
Dong Hyeon Shin; Departments of Internal Medicine, Chonnam National University Medical School, Gwangju, Korea
Sook In Jung; Departments of Internal Medicine, Chonnam National University Medical School, Gwangju, Korea
Young Kyu Park; Departments of General Surgery, Chonnam National University Medical School, Gwangju, Korea
Myung Gun Shin; Departments of Laboratory Medicine, Chonnam National University Medical School, Gwangju, Korea
Duck Cho; Departments of Laboratory Medicine, Chonnam National University Medical School, Gwangju, Korea
Seong Jung Kee; Departments of Laboratory Medicine, Chonnam National University Medical School, Gwangju, Korea
Soon Pal Suh; Departments of Laboratory Medicine, Chonnam National University Medical School, Gwangju, Korea
Dong Wook Ryang; Departments of Laboratory Medicine, Chonnam National University Medical School, Gwangju, Korea

Corresponding

Jong Hee Shin, Departments of Laboratory Medicine, Chonnam National University Medical School, Gwangju, Korea. Tel: (062) 220-5342, Fax: (062) 224-2518, e-mail: shinjh@chonnam.ac.kr

Publication history

Acknowledgements

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

Jong Hee Shin

Departments of Laboratory Medicine, Chonnam National University Medical School, Gwangju, Korea

Mira Park

Departments of Laboratory Medicine, Chonnam National University Medical School, Gwangju, Korea

Jung Won Song

Departments of Laboratory Medicine, Chonnam National University Medical School, Gwangju, Korea

Dong Hyeon Shin

Departments of Internal Medicine, Chonnam National University Medical School, Gwangju, Korea

Sook In Jung

Departments of Internal Medicine, Chonnam National University Medical School, Gwangju, Korea

Young Kyu Park

Departments of General Surgery, Chonnam National University Medical School, Gwangju, Korea

Myung Gun Shin

Departments of Laboratory Medicine, Chonnam National University Medical School, Gwangju, Korea

Duck Cho

Departments of Laboratory Medicine, Chonnam National University Medical School, Gwangju, Korea

Seong Jung Kee

Departments of Laboratory Medicine, Chonnam National University Medical School, Gwangju, Korea

Soon Pal Suh

Departments of Laboratory Medicine, Chonnam National University Medical School, Gwangju, Korea

Dong Wook Ryang

Departments of Laboratory Medicine, Chonnam National University Medical School, Gwangju, Korea

Since epub date 2016 February 20