Abstract

Various oxidases and hydrolytic enzymes were analyzed to investigate the relationship between these enzymes and the skin pathogenicity of 18 Mucorales strains. Each strain was cultured in a nutrient medium containing starch as a carbon source. The cells grew quickly and were at a good state of growth after incubation for three days. Oxidase activity was not detected in any strain, whereas Mucor spp. including Mucor racemosus IFM47053 typically had high alcohol dehydrogenase (ADH) activity and all the strains had catalase activity. The culture filtrate and the cell free extract of each strain were applied to APIZYM test system, which revealed that all the strains examined produced many hydrolytic

enzymes both inside and outside their mycelia. In the case of Absidia corymbifera strains, lipase activity was comparatively high, and polysaccharide hydrolytic enzymes such as α-glucosidase, β-glucosidase, N-acetyl-β-glucosaminidase, α-mannosidase, and α-fucosidase were produced.

Keywords

Zygomycosis Alcohol dehydrogenase (ADH) Catalase Hydrolases

KJMM 2007 March;12(1):9-17(9). Epub 2016 February 19
Copyright © 2007 by Korean Journal of Medical Mycology

Language

English

Author

Hee Sun Ko; Center for Traditional Microorganism Resources, Keimyung University, Dalseo-gu, Daegu 704-701, Korea
Hideaki Taguchi; Research Center for Pathogenic Fungi and Microbial Toxicoses, Chiba University, Chiba 260-8673, Japan
Kayoko Takizawa; Research Center for Pathogenic Fungi and Microbial Toxicoses, Chiba University, Chiba 260-8673, Japan
Kazutaka Fukusima; Research Center for Pathogenic Fungi and Microbial Toxicoses, Chiba University, Chiba 260-8673, Japan
Hyun Soo Kim; Center for Traditional Microorganism Resources, Keimyung University, Dalseo-gu, Daegu 704-701, Korea; Department of Microbiology, Collage of Natural Science, Keimyung University, Dalseo-gu, Daegu 704-701, Korea

Corresponding

Hyun Soo Kim, Department of Microbiology, Collage of Natural Science, Keimyung University, Dalseo-gu, Daegu 704-701, Korea. Tel: +82-(0)53-580-5284, Fax: +82-(0)53-580-5509, e-mail: hskim@kmu.ac.kr

Publication history

Acknowledgements

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

Hee Sun Ko

Center for Traditional Microorganism Resources, Keimyung University, Dalseo-gu, Daegu 704-701, Korea

Hideaki Taguchi

Research Center for Pathogenic Fungi and Microbial Toxicoses, Chiba University, Chiba 260-8673, Japan

Kayoko Takizawa

Research Center for Pathogenic Fungi and Microbial Toxicoses, Chiba University, Chiba 260-8673, Japan

Kazutaka Fukusima

Research Center for Pathogenic Fungi and Microbial Toxicoses, Chiba University, Chiba 260-8673, Japan

Hyun Soo Kim

Center for Traditional Microorganism Resources, Keimyung University, Dalseo-gu, Daegu 704-701, Korea; Department of Microbiology, Collage of Natural Science, Keimyung University, Dalseo-gu, Daegu 704-701, Korea

Since epub date 2016 February 19