Abstract

Hexane, dichloromethane, methanol and water extracts from Galla rhois were examined for antifungal activity against Candida albicans isolated from the patients with recurrent vaginal candidiasis. Only methanol extract showed anticandidal activity and the methanol extract was fractionated through silica gel column. Each fraction had not antifugal activity but mixture of three fractions showed antifungal activity. The results showed that the methanol extract from Galla rhois had antifungal activity against Candida albicans and could be a candidate for new antifungal agent.

Background: Candida albicans is a member of the normal flora of the skin, mucous membranes and gastrointestinal tract. Vaginal candidiasis remains a significant problem in women of childbearing age, majority of the cases are caused by C. albicans and recurrence is common in spite of topical treatment.

Objective: The purpose of this study was to develop the antifungal agent from the medicinal herbs traditionally used in Korea.

Method: In this study, the extracts from Galla rhois were examined for antifungal activities against C. albicans. Galla fhois was extracted with hexane, dichloromethane, methanol and water respectively and serially. Silica gel column chromatography and thin layer chromatography were used to separate the fractions with antifungal activity.

Results: Only methanol extract showed the antifungal activity aginst C. albicans.

Conclusion: Methanol extract from Galla rhois could be a candidate for a new antifungal agent against C. albicans.

Keywords

Galla rhois Methanol extract Antifungal activity Candida albicans

KJMM 2007 December;12(4):175-179(5). Epub 2016 February 19
Copyright © 2007 by Korean Journal of Medical Mycology

Language

Korean/English

Author

Inwha Seong; Department of Microbiology, College of Medicine, Korea University, Seoul 136-705, Republic of Korea

Corresponding

Inwha Seong, Department of Microbiology, College of Medicine, Korea University, Seoul 136-705, Republic of Korea. Tel: (02) 920-6167, Fax: (02) 923-3645, e-mail: inwha@korea.ac.kr

Publication history

Acknowledgements

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

Inwha Seong

Department of Microbiology, College of Medicine, Korea University, Seoul 136-705, Republic of Korea

Since epub date 2016 February 19