Abstract

Background: A standardized reference method for dermatophytes in vitro susceptibility testing is lacking. However, with increasing variety of drugs available to treat dermatophytosis, the need for a reference method for dermatophytes testing has become apparent.

Objective: To evaluate a method of quantifying dermatophytes, the standards for broth microdilution method and evaluation of the availability of disk diffusion method in antifungal susceptibility testing for dermatophytes.

Methods: 14 Candida species (sp.), 30 Trichophyton(T.) mentagrophytes, 9 T. raubitschekii and 11 T. rubrum were tested for fluconazole susceptibility by the broth microdilution method and disc diffusion method. Candida sp. was tested according to National Committee for Clinical Laboratory Standards (NCCLS) M27-A and M44-A. Broth microdilution method for T. mentagrophytes, T. raubitschekii and T. rubrum was operated according to NCCLS M38-A. Disk diffusion method for T. mentagrophytes, T. raubitschekii and T. rubrum was tested refer to NCCLS M44-A.

Result: The disk diffusion method showed 50% correlation rate with the broth microdilution method for antifungal susceptibility testing for Candida species. The MIC reading point and incubation time of the broth microdilution method for T. mentagrophytes, T. raubitschekii and T. rubrum are Spec-50 and from 7, 6 and 9 days. Relation between the broth microdilution method and disk diffusion method for dermatophytes is poor.

Conclusions: The good method for quantifying dermatophytes is using vortexing only or liquid nitrogen and homogenizer. Standards of MIC reading point and incubation time of microdilution method for dermatophytes are Spec-50 and from 6 to 9 days. It appears that the disk diffusion method is not recommended method for the antifungal susceptibility testing of dermatophytes.

Keywords

Antifungal susceptibility test Dermatophytes Disk diffusion method Microdilution method

KJMM 2008 June;13(2):61-74(14). Epub 2016 February 19
Copyright © 2008 by Korean Journal of Medical Mycology

Language

Korean/English

Author

Seok Ki Moon; Department of Dermatology, College of Medicine, Yeungnam University, Daegu, Korea
Dong Hoon Shin; Department of Dermatology, College of Medicine, Yeungnam University, Daegu, Korea
Jong Soo Choi; Department of Dermatology, College of Medicine, Yeungnam University, Daegu, Korea
Ki Hong Kim; Department of Dermatology, College of Medicine, Yeungnam University, Daegu, Korea
Keuk Jun Kim; Department of Pathology, College of Medicine, Yeungnam University, Daegu, Korea

Corresponding

Jong Soo Choi, Department of Dermatology, College of Medicine, Yeungnam University, Daegu, Korea. Tel: (053) 620-3160, Fax: (053) 622-2216, e-mail: jschoi@med.yu.ac.kr

Publication history

Acknowledgements

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

Seok Ki Moon

Department of Dermatology, College of Medicine, Yeungnam University, Daegu, Korea

Dong Hoon Shin

Department of Dermatology, College of Medicine, Yeungnam University, Daegu, Korea

Jong Soo Choi

Department of Dermatology, College of Medicine, Yeungnam University, Daegu, Korea

Ki Hong Kim

Department of Dermatology, College of Medicine, Yeungnam University, Daegu, Korea

Keuk Jun Kim

Department of Pathology, College of Medicine, Yeungnam University, Daegu, Korea

Since epub date 2016 February 19