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Protective Effects of Immunization with Proteinase, Phospholipase and Cell Wall Proteins of Candida albicans in Mice Candidosis

Woon-Seob Shin,Sun Ju Choi,Young Chul Yang,Su Jung Park,Joo Young Park,Soo-Ki Kim,Kyoung-Ho Lee
Epub 2016 February 18

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Abstract



Background: The opportunistic fungus Candida albicans is a major pathogen especially to immunocompromised patients.


Objectives: We examined the protective effect of the active and passive immunizations to evaluate the applicability for the treatment of candidosis in Candida-infected mice model.


Methods: Candida cell wall components were obtained by treatment of lyticase, proteinase K, and dithiothreitol. The proteinase was purified from the culture filtrates of C. albicans using a series of chromatographic steps consisting of DEAE-Sepharose FF, Sephacryl S-200 HR and size-exclusion high performance liquid chromatography. The phospholipase was purified from the culture supernatant of C. albicans with DEAE column chromatography, reverse phase column chromatography, revere phase HPLC and size-exclusion HPLC. Antibodies to cell wall protein components, proteinase and phospholipase were produced by immunization into mice of same strain.


Results: The mean survival times of active and passive immunized mice groups were longer than those of non-immunized groups.


Conclusion: These results showed that immunization with proteinase and its antibody were the most effective to prolong survival time in Candida-infected mice.



Keywords


Candida albicans Proteinase Phospholipase Immunization




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