본문바로가기

Reidentification of Aspergillus spp. Isolated from Clinical Specimens of Patients Suspected as Pulmonary Aspergillosis in Korea

Dae-Ho Kim,Sung-Hee Kim,Young-Kwon Kim,Shin-Ok Kim,Sang-Jae Kim,Seung-Beom Hong
Epub 2016 February 18

0
Cited By

Abstract



Korean Institute of Tuberculosis (KIT) collected 129 strains of Aspergillus spp. from clinical specimens of patient suspected as pulmonary Aspergillosis. On the basis of morphological characteristics, these strains were identified as A. fumigatus (62 strains), A. niger (37), A. flavus (26), A. versicolor (1), A. nidulans (1), A. clavatus (1) and Neosartorya fennelliae (1). These strains were re-identified according to recent Aspergillus classification system which is mainly based on molecular characters. The strains were grouped by Randomly Amplified Polymorphic DNA (RAPD). The representative strains from each group were sequenced with partial β-tubulin gene and compared with those of reference strains in the Aspergillus and were identified by the sequence. The identification was confirmed by morphological examination. As the results, they are reidentified as A. fumigatus (58), A. niger (11), A. tubingensis (26),

A. flavus (27), A. sydowii (3), A. nidulans (1), A. clavatus (1), Neosartorya fennelliae (1), N. fischeri (1). This is also the first report of A. tubuingensis in clinical field in Korea.



Keywords


Plumonary Aspergillosis Aspergillus Identification β-tubulin




Congratulatory MessageClick here!

Download this article
Submit your research
Jump to: