Abstract

BACKGROUND: The early diagnosis of invasive candidiasis is the most important for reducing of morbidity and mortality rates in the immunocompromised patients. The study of antigen detection was performed by Cand-Tec kit, but antibody detection by Western blot, had not been reported.

OBJECTIVE: We reviewed 62 cases of suspected invasive candidiasis (10), immunocompromised patients with leukemia, leukopenia, pneumonia, et al (44) and normal colonization control (8) in the urine and sputum for the immunological diagnosis of invasive candidiasis by Cand-Tec and Western blot assays.

METHODS: The antigen and antibody detection were done by both Cand-Tec kit and Western blot assay, in the sera of the patients collected from several hospitals.

RESULTS: The sera from 4 (40%) and 8 (80%) of 10 suspected invasive candidiasis had a threshold positive titers of ≥ 1:4 by latex agglutination (Cand-Tec) and the results of positive antibody to the immunodominant antigen (47 kDa) of Candida spp. by Western blot assay, each other. Both antigen and antibody we.e detected from 21 (47%) of 44 patients in the immunocompromised group and 1 (12%) of 8 cases with normal flora, separately, but the antibodies from 3 (18%) of 16 healthy controls were detected by Western blot analysis, only.

CONCLUSION: This study confirm that the Western blot method of detecting antibody against immunodominant antigen (47 KDa) of Candida spp. was more effective than antigen detection by Cand-Tec method for immunodiagnosis of invasive candidiasis.

Keywords

Cand-Tec Invasive candidiasis Western blot

KJMM 1996 December;1(1):55-62(8). Epub 2016 February 25
Copyright © 1996 by Korean Journal of Medical Mycology

Language

Korean/English

Author

Bong Su Kim; Laboratory of Nosocomial Pathogens, Department of Microbial Diseases, National Institute of Health, Seoul, Korea
Yeong Seon Lee; Laboratory of Nosocomial Pathogens, Department of Microbial Diseases, National Institute of Health, Seoul, Korea
In Seon Cho; Laboratory of Nosocomial Pathogens, Department of Microbial Diseases, National Institute of Health, Seoul, Korea
Dong Han Kim; Laboratory of Nosocomial Pathogens, Department of Microbial Diseases, National Institute of Health, Seoul, Korea
Kee Duk Park; Laboratory of Nosocomial Pathogens, Department of Microbial Diseases, National Institute of Health, Seoul, Korea
Jong Hee Shin; Chonnam University Medical School, Kwangju, Korea

Corresponding

Bong Su Kim, Laboratory of Nosocomial Pathogens, Department of Microbial Diseases, National Institute of Health, Seoul, Korea.

Publication history

Acknowledgements

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

Bong Su Kim

Laboratory of Nosocomial Pathogens, Department of Microbial Diseases, National Institute of Health, Seoul, Korea

Yeong Seon Lee

Laboratory of Nosocomial Pathogens, Department of Microbial Diseases, National Institute of Health, Seoul, Korea

In Seon Cho

Laboratory of Nosocomial Pathogens, Department of Microbial Diseases, National Institute of Health, Seoul, Korea

Dong Han Kim

Laboratory of Nosocomial Pathogens, Department of Microbial Diseases, National Institute of Health, Seoul, Korea

Kee Duk Park

Laboratory of Nosocomial Pathogens, Department of Microbial Diseases, National Institute of Health, Seoul, Korea

Jong Hee Shin

Chonnam University Medical School, Kwangju, Korea

Since epub date 2016 February 25