Abstract

BACKGROUND: Several groups of workers have isolated and purified the keratinolytic proteinases from some species of dermatophytoses. But it is not efficient to compare the interspecies of dermatophytosis because the separation and purification are done by different conditions.

OBJECTIVE: To compare the characteristics and difference of the keratinases which are isolated and purified from Trichophyton rubrum, Trichophyton mentagrophytes and Microsporum canis in the same conditions.

METHODS: Proteinases elaborated from T. rubrum, T. mentagrophytes and M. canis were purified by precipitation using ammonium sulfate and by SMART. SDS-polyacrylamide gel electrophoresis was carried out, and the effect of various inhibitors was investigated. A variety of substrates for KPase were tested.

RESULTS:

1. The keratinases were isolated and purified 17.3, 22.0, 17.6-fold from T. rubrum, T. mentagrophytes and M. canis.

2. Each molecular weight of the keratinolytic proteinases isolated and purified from T. rubrum, T. mentagrophytes and M. canis was 53 kDa, 65 kDa, 77 kDa.

3. Phenylmethylsulfonylfluoride inhibited the keratinase activity in T. rubrum, T. mentagrophytes and M. canis.

4. The keratinases from T. rubrum, T. mentagrophytes and M. canis digested much more human stratum corneum and human nail than human scalp hair, but keratinase from M. canis digested much more human scalp hair than human stratum corneum and human nail.

CONCLUSION: The results show keratinases from T. rubrum, T. mentagrophytes and M. canis were different each other, but they may be a kind of serine proteinase. The keratinases from T. rubrum, T. mentagrophytes and M. canis may play an important role in tinea pedis and tinea unguis by affecting the human stratum corneum and human nail, and the keratinase from M. canis may play an important role in tinea capitis by affecting the human hair. 

Keywords

Keratinase Microsporum canis SMART Trichophyton mentagrophytes Trichophyton rubrum

KJMM 1998 December;3(2):95-106(12). Epub 2016 February 24
Copyright © 1998 by Korean Journal of Medical Mycology

Language

Korean/English

Author

Sang-Ju Lee; Department of Dermatology, Yonsei University College of Medicine, Seoul, Korea
Kwang Hoon Lee; Department of Dermatology, Yonsei University College of Medicine, Seoul, Korea
Won Jae Lee; Department of Laboratory of Immunology, Medical Research Center, Seoul, Korea
Jung Bock Lee; Department of Dermatology, Yonsei University College of Medicine, Seoul, Korea

Corresponding

Jung Bock Lee, Department of Dermatology, Yonsei University College of Medicine, Seoul, Korea.

Publication history

Acknowledgements

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

Sang-Ju Lee

Department of Dermatology, Yonsei University College of Medicine, Seoul, Korea

Kwang Hoon Lee

Department of Dermatology, Yonsei University College of Medicine, Seoul, Korea

Won Jae Lee

Department of Laboratory of Immunology, Medical Research Center, Seoul, Korea

Jung Bock Lee

Department of Dermatology, Yonsei University College of Medicine, Seoul, Korea

Since epub date 2016 February 24