Abstract

Background: It is necessary to perform antifungal susceptibility testing of dermatophytes. Etest (AB Biodisk, Sweden) is a rapid, easy-to-perform in-vitro antifungal susceptibility test.

Objective: The purpose of this study was to investigate the minimal inhibitory concentration (MICs) of dermatophytes isolated from skin using Etest.

Methods: 21 species of dermatophytes (12 strains of T. rubrum, 7 strains of T. mentagrophytes, M. canis and M. gypseum) and two standard strains (Aspergillus flavus KCTC 6905, Aspergillus fumigatus KCTC 6145) were tested MIC endpoints of Etest for itraconazole (ITZ) and amphotericin B (AMB) were read after 72, 96, and 120 hours incubation for each strains on RPMI 1640 agar.

Results: MIC of ITZ was 0.12~0.47 μg/mL on T. rubrum, 0.012~1.0 μg/mL on T. mentagrophytes, 0.012 μg/mL on M. canis, and 0.023 μg/mL on M. gypseum. MIC of AMB was 0.094~0.5 μg/mL on T. rubrum, 0.032~1.0 μg/mL on T. mentagrophytes, 0.19 μg/mL on M. canis, and 0.032 μg/mL on M. gypseum. One strain of T. mentagrophytes isolated from patient with tinea pedis showed ITZ-resistant.

Conclusion: This study showed that Etest represented a simple and efficacious method for antifungal susceptibility testing of dermatophytes.

Keywords

Etest Dermatophytes

KJMM 2010 September;15(3):124-133(10). Epub 2016 February 18
Copyright © 2010 by Korean Journal of Medical Mycology

Language

Korean/English

Author

Gyo Shin Kang; Departments of Dermatology, College of Medicine, Dongguk University, Gyeongju, Korea
Moo Kyu Suh; Departments of Dermatology, College of Medicine, Dongguk University, Gyeongju, Korea
Gyoung Yim Ha; Departments of Laboratory Medicine, College of Medicine, Dongguk University, Gyeongju, Korea

Corresponding

Moo Kyu Suh, Departments of Dermatology, College of Medicine, Dongguk University, Gyeongju, Korea. Tel: (054) 770-8268, Fax: (054) 773-1581, e-mail: smg@dongguk.ac.kr

Publication history

Received 4 July 2010; Revised 28 August 2010; Accepted 30 August 2010.

Acknowledgements

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

Gyo Shin Kang

Departments of Dermatology, College of Medicine, Dongguk University, Gyeongju, Korea

Moo Kyu Suh

Departments of Dermatology, College of Medicine, Dongguk University, Gyeongju, Korea

Gyoung Yim Ha

Departments of Laboratory Medicine, College of Medicine, Dongguk University, Gyeongju, Korea

Since epub date 2016 February 18